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. 2009 Aug 3;119(9):2758–2771. doi: 10.1172/JCI39162

Figure 6. Sirt3 blocks the agonist-induced signaling pathways involved in development of cardiac hypertrophy.

Figure 6

(A) Rat cardiomyocytes were infected with Ad.Sirt3 or Ad.Smut viruses and treated with PE (20 μM) for the indicated time intervals (0, 5, and 60 minutes). Cell lysate was analyzed by Western blotting with kinase-specific and phospho-kinase–specific antibodies. Numbers in parentheses indicate the position of the phospho–amino acid recognized by the antibody. (B) ERK1/2 phosphorylation was analyzed 24 hours after PE-treatment of cells. (C) Heart extract prepared from N-Tg and Sirt3-Tg mice subjected to Ang II–mediated hypertrophy was analyzed by Western-blotting with antibodies against different kinases as indicated. Results are shown for 2 mice of each group.