Table 4.
Expected mean squared errors for option 2 of the combined model.Table shows the expected mean squared errors associated with a combined model. This layout corresponds to a 2 × 2 × 5 factorial arrangement of sex, time, AZT and their interactions with the 60 pooled muscle samples (2 samples per pool) processed in 12 batches (blocks); each batch has 5 pools, one pool for each AZT level within a sex and a sacrifice time.
| Source | DF | Expected mean square (approx.) |
|---|---|---|
| Mean: Housekeeping genes | 1 | σ2 + σ2m + Q(Slope) |
| Sex | 1 | σ2 + σ2m + 10σ2b + Q(Sex) |
| Time | 1 | σ2 + σ2m + 10σ2b + Q(Time) |
| Sex * Time | 1 | σ2 + σ2m + 10σ2b + Q(Sex * Time) |
| Batch | 8 | σ2 + σ2m + 10σ2b |
| AZT | 4 | σ2 + σ2m + 10σ2b + Q(AZT) |
| AZT * Sex | 4 | σ2 + σ2m + 10σ2b + Q(AZT * Sex) |
| AZT * Time | 4 | σ2 + σ2m + 10σ2b + Q(AZT * Time) |
| AZT * Sex * Time Time) | 4 | σ2 + σ2m + 10σ2b + Q(AZT * Sex * |
| Mice within treatment | 31 | σ2 + σ2m |