Table III.

Amino acid accumulation in rosette leaves of the wild type, the nadk2 mutant, and NADK2 overexpression (NADK2-OX1 and -OX2) lines

Values indicate means obtained from three independent experiments. se values are in all cases less than 35% of mean values. Statistical significances relative to the wild type by t test are as follows: * P < 0.01, ** P < 0.05, *** P < 0.1

Metabolite	Concentration
	Wild Type	nadk2	NADK2-OX1	NADK2-OX2
	nmol g−1 fresh weight
Asp family
Asn	1,618.4 ± 54.7	671.7 ± 54.9**	2,074.2 ± 234.0	1,582.9 ± 350.5
Asp	1,238.4 ± 180.1	2,944.0 ± 477.6***	1,628.5 ± 168.9	1,719.6 ± 170.8
Lys	106.5 ± 13.9	50.2 ± 5.2**	90.9 ± 6.4	81.0 ± 23.4
Met	14.9 ± 3.0	7.4 ± 0.2***	19.0 ± 1.3	16.6 ± 0.4
Thr	635.5 ± 43.1	374.6 ± 22.5**	922.6 ± 53.6**	951.1 ± 104.6***
Glu family
Arg	4,519.7 ± 581.6	443.4 ± 75.5**	5,890.5 ± 382.0**	6,375.7 ± 1,258.9
Gln	4,236.9 ± 537.0	1,071.5 ± 55.6**	6,490.2 ± 546.7*	6,555.0 ± 813.8***
Glu	2,786.6 ± 265.3	2,460.3 ± 519.2	3,917.8 ± 190.5**	4,388.1 ± 142.8**
Pro	984.1 ± 99.7	137.6 ± 9.1*	2,070.1 ± 641.2	1,075.5 ± 251.4
Ser family
Cys	4.8 ± 0.6	13.1 ± 1.1**	7.6 ± 0.2**	9.1 ± 1.9
Gly	259.6 ± 72.1	113.2 ± 14.1***	485.1 ± 56.7**	282.9 ± 80.4
Ser	3,961.0 ± 272.0	2,971.5 ± 172.4**	5,069.3 ± 40.9**	4,300.2 ± 211.1
Pyruvate family
Ala	271.5 ± 54.7	822.2 ± 129.8**	368.5 ± 49.9	385.6 ± 29.2**
Leu	75.2 ± 14.7	69.3 ± 5.9	66.9 ± 6.7	58.0 ± 2.5
Val	97.1 ± 12.1	116.7 ± 12.6	114.1 ± 12.6	102.3 ± 5.5
Aromatic amino acids
Phe	36.3 ± 6.0	36.3 ± 3.9	45.7 ± 2.1	35.2 ± 2.5
Trp	4.6 ± 1.2	8.6 ± 0.7***	4.8 ± 0.9	6.3 ± 1.0
Tyr	15.1 ± 1.8	12.6 ± 0.5	16.2 ± 1.6	12.7 ± 0.8
His family
His	116.7 ± 9.4	78.8 ± 9.7***	130.5 ± 12.4	108.6 ± 17.7
Total	21,027.8 ± 1,867.6	12,425.6 ± 1,014.1**	29,502.4 ± 482.6**	28,124.8 ± 1,927.1***