Figure 5.

The RbcS-tp T5 segment confers Toc159 dependency to protein import of E1α-tp. A, Sequence of hybrid TPs of E1α-tp and RbcS-tp. The T2, T3, T4, T5, or T6 segment of E1α-tp was replaced with the corresponding segment from RbcS-tp. B, Western-blot analysis of import experiments. Protein extracts from wild-type or ppi2 protoplasts transformed with the indicated constructs were used for western-blot analysis using an anti-GFP antibody. The import experiments were performed three times. Pre, Precursor forms; Pro, processed forms. C, Quantification of import efficiency. The intensities of the bands shown in B were quantified as described in Figure 1F and the data shows the average import efficiency (n = 3; error bars, sd). D, Complementation analysis of E1α[RbcST5]-tp. E1α[RbcST5]-tp was cotransformed with R6 or T7:atTOC159 into ppi2 protoplasts and the protoplasts were analyzed by western-blot analysis with anti-GFP antibody.