Figure 2.

Expression profiles of genes involved in CK perception in maize leaves (A) and roots (B) exposed to osmotic stress (dark gray bars), salinity stress (light gray bars), and exogenous BAP application (white bars). Total RNA was isolated from tissues exposed to stress and CK stimuli at six time points from at least two independent biological replicates. cDNA from each sample was obtained at least in two independent reactions and run in two separate PCRs. The actin gene was used as an endogenous control, and untreated tissue samples were used as calibrators (black bars) in which expression level was set to 1. Only genes with abundance above one copy per 1 ng of total isolated RNA are included.