Table I.
Transcript abundance in different maize organs
Abundance is expressed as gene copy number in 1 ng of total RNA amplified by qPCR, normalized to 18S RNA, and recalculated as primer pair efficiency. Total RNA from each sample was transcribed in two independent reactions, and PCR was performed in duplicate. Mean values with sd are shown.
| Gene | Embryo | Endosperm | Pedicel | Silk | Tassel | Immature Ear | Coleoptile + Radicle | 7-d-Old Root | Mature Root | 7-d-Old Leaf | Mature Leaf |
|---|---|---|---|---|---|---|---|---|---|---|---|
| IPT genes | |||||||||||
| IPT1 | 101.9 ± 33.6 | 110.8 ± 6.6 | 83.4 ± 5.4 | 112.3 ± 12.9 | 111.9 ± 4.8 | 114.2 ± 17.8 | 63.0 ± 8.9 | 71.6 ± 12.0 | 111.2 ± 14.9 | 125.0 ± 17.8 | 38.8 ± 10.6 |
| IPT2 | 10.9 ± 2.2 | 89.4 ± 13.8 | 303.3 ± 17.2 | <1.0 | <1.0 | <1.0 | <1.0 | <1.0 | <1.0 | <1.0 | <1.0 |
| IPT3 | <1.0 | <1.0 | 1.1 ± 0.3 | <1.0 | 2.9 ± 0.6 | 1.2 ± 0.2 | <1.0 | <1.0 | 3.5 ± 0.2 | <1.0 | 1.1 ± 0.7 |
| IPT3b | <1.0 | <1.0 | <1.0 | <1.0 | 1.1 ± 0.2 | <1.0 | <1.0 | <1.0 | <1.0 | <1.0 | <1.0 |
| IPT4 | 1.9 ± 0.5 | <1.0 | <1.0 | <1.0 | <1.0 | <1.0 | <1.0 | 1.0 ± 0.4 | 8.3 ± 0.3 | <1.0 | <1.0 |
| IPT5 | 5.1 ± 0.7 | <1.0 | 34.5 ± 6.2 | 32.3 ± 4.2 | 353.7 ± 29.0 | 66.4 ± 13.7 | 3.7 ± 1.0 | 49.7 ± 12.5 | 169.3 ± 7.9 | 14.0 ± 9.3 | 33.2 ± 4.9 |
| IPT6 | <1.0 | 1.7 ± 0.6 | <1.0 | 2.1 ± 0.4 | 8.5 ± 2.1 | 1.6 ± 0.3 | 1.1 ± 0.2 | 8.3 ± 2.7 | 19.2 ± 4.5 | <1.0 | <1.0 |
| IPT7 | <1.0 | <1.0 | 2.1 ± 0.2 | <1.0 | 11.2 ± 2.3 | <1.0 | <1.0 | 1.6 ± 0.4 | <1.0 | <1.0 | <1.0 |
| IPT8 | 8.5 ± 1.1 | 35.8 ± 3.8 | 4.2 ± 1.1 | 3.0 ± 0.5 | 7.3 ± 4.2 | <1.0 | 1.4 ± 0.9 | <1.0 | <1.0 | 6.4 ± 1.4 | 8.8 ± 0.9 |
| IPT9 | 2.2 ± 1.2 | <1.0 | 3.2 ± 0.7 | <1.0 | 7.9 ± 1.2 | <1.0 | <1.0 | 2.7 ± 0.6 | 23.2 ± 3.6 | 2.7 ± 0.3 | <1.0 |
| IPT10 | 51.5 ± 2.3 | 25.1 ± 1.6 | 16.7 ± 1.3 | 8.9 ± 0.6 | 40.9 ± 3.1 | 19.7 ± 2.8 | 48.0 ± 3.4 | 35.9 ± 2.7 | 11.2 ± 3.6 | 47.7 ± 1.0 | 46.9 ± 2.4 |
| CK oxidase/dehydrogenase genes | |||||||||||
| CKX1 | 422.9 ± 13.5 | 37.2 ± 7.4 | 95.2 ± 12.0 | 73.6 ± 14.0 | 190.0 ± 31.7 | 87.1 ± 15.5 | 2.1 ± 0.2 | 54.3 ± 4.5 | 116.7 ± 5.4 | 1.6 ± 0.1 | 1.5 ± 0.1 |
| CKX2 | 23.2 ± 2.7 | 35.8 ± 2.8 | 78.8 ± 8.4 | 19.4 ± 2.5 | 224.6 ± 12.6 | 20.9 ± 1.8 | 6.2 ± 0.7 | 18.7 ± 2.1 | 37.1 ± 1.6 | 27.5 ± 3.7 | 609.4 ± 24.2 |
| CKX3 | 15.3 ± 1.9 | 53.7 ± 0.7 | 38.7 ± 4.2 | 65.2 ± 6.8 | 12.4 ± 1.0 | 36.6 ± 2.2 | 2.9 ± 0.3 | 25.6 ± 0.9 | 5.8 ± 0.2 | 160.4 ± 5.3 | 10.2 ± 0.6 |
| CKX4 | <1.0 | 1.6 ± 0.7 | 57.1 ± 5.1 | 13.9 ± 0.4 | 2.5 ± 0.4 | 2.6 ± 0.1 | <1.0 | 2.6 ± 0.1 | 11.1 ± 0.2 | 1.1 ± 0.1 | 3.8 ± 0.2 |
| CKX4b | 7.9 ± 0.4 | <1.0 | 37.7 ± 6.5 | 21.7 ± 2.0 | 17.4 ± 3.8 | 7.4 ± 1.2 | 2.0 ± 0.2 | 26.3 ± 2.8 | 763.9 ± 96 | 20.3 ± 4.6 | 21.0 ± 1.8 |
| CKX5 | <1.0 | 1.8 ± 0.3 | 7.4 ± 2.0 | 54.6 ± 2.4 | 23.5 ± 1.2 | 2.5 ± 0.6 | <1.0 | <1.0 | 6.1 ± 0.4 | <1.0 | <1.0 |
| CKX6 | 16.4 ± 3.6 | <1.0 | 32.2 ± 3.3 | 4.2 ± 0.3 | 74.0 ± 3.4 | 16.6 ± 1.2 | 38.0 ± 1.7 | 221.2 ± 12.0 | 99.1 ± 11.0 | 93.4 ± 7.0 | 20.4 ± 1.7 |
| CKX7 | <1.0 | <1.0 | <1.0 | <1.0 | <1.0 | <1.0 | <1.0 | <1.0 | <1.0 | <1.0 | <1.0 |
| CKX8 | 6.6 ± 0.5 | <1.0 | <1.0 | <1.0 | <1.0 | <1.0 | 2.1 ± 0.3 | 38.0 ± 19.6 | 2.6 ± 0.1 | 1.1 ± 0.2 | 4.6 ± 0.7 |
| CKX9 | <1.0 | <1.0 | <1.0 | <1.0 | <1.0 | 1.3 ± 0.1 | <1.0 | <1.0 | <1.0 | 1.8 ± 0.7 | <1.0 |
| CKX10 | 141.6 ± 1.1 | 13.3 ± 0.7 | 229.3 ± 30.2 | 647.2 ± 56.1 | 1,120.1 ± 48.9 | 351.2 ± 28.7 | 219.2 ± 18.4 | 61.2 ± 18.4 | 126.2 ± 10.4 | 310.9 ± 25.1 | 76.6 ± 18.0 |
| CKX11 | 2.0 ± 0.1 | <1.0 | <1.0 | 1.0 ± 0.2 | 3.5 ± 0.3 | 1.3 ± 0.3 | 1.8 ± 0.1 | 2.5 ± 0.3 | 4.6 ± 0.3 | 71.7 ± 4.6 | 2.9 ± 0.7 |
| CKX12 | 9.5 ± 2.7 | 13.4 ± 0.8 | <1.0 | <1.0 | 3.8 ± 1.6 | <1.0 | 1.5 ± 0.5 | 2.4 ± 0.2 | 2.7 ± 0.4 | 10.3 ± 0.6 | 3.5 ± 1.9 |
| CK receptor genes | |||||||||||
| HK1 | 1,712.9 ± 40.9 | 821.7 ± 38.7 | 1,117.1 ± 54.8 | 1,243.1 ± 42.0 | 1,439.8 ± 45.8 | 2,237.4 ± 75.9 | 838.2 ± 45.4 | 2,078.9 ± 45.6 | 763.4 ± 27.5 | 596.3 ± 30.5 | 322.6 ± 17.5 |
| HK2 | 668.2 ± 47.0 | 818.8 ± 61.5 | 573.1 ± 29.5 | 1,849.3 ± 149.2 | 2,344.8 ± 88,3 | 2,233.0 ± 155.0 | 1,166.6 ± 147.0 | 4,812.8 ± 202.2 | 1,095.5 ± 85.7 | 2,394.4 ± 102.5 | 1,322.8 ± 147.8 |
| HK3 | 141.0 ± 9.8 | 340.4 ± 41.5 | 158.0 ± 25.4 | 362.5 ± 36.6 | 273.1 ± 30.0 | 225.1 ± 17.7 | 178.2 ± 18.2 | 641.5 ± 54.4 | 195.6 ± 9.8 | 150.8 ± 7.9 | 240.6 ± 13.2 |
| CK response regulator type A genes | |||||||||||
| RR1 | <1.0 | <1.0 | <1.0 | <1.0 | <1.0 | <1.0 | 55.5 ± 4.7 | 189.4 ± 32.4 | <1.0 | 449.1 ± 19.7 | <1.0 |
| RR2 | 5.6 ± 1.4 | <1.0 | 29.7 ± 3.8 | <1.0 | 11.2 ± 0.8 | 4.3 ± 0.3 | 38.7 ± 7.2 | 116.4 ± 11.7 | 19.1 ± 3.7 | 66.6 ± 5.8 | 92.9 ± 24.0 |
| RR3 | 16.8 ± 2.7 | <1.0 | <1.0 | <1.0 | <1.0 | 3.3 ± 0.2 | <1.0 | <1.0 | <1.0 | 0.3 ± 0.1 | <1.0 |
| RR4 | 118.2 ± 24.7 | 20.3 ± 3.0 | 201.7 ± 41.0 | 39.2 ± 4.9 | 435.3 ± 33.7 | 112.6 ± 6.7 | 615.6 ± 47.5 | 979.5 ± 54.5 | 190.8 ± 5.6 | 303.5 ± 24.2 | 356.0 ± 26.8 |
| RR5 | 36.8 ± 9.1 | 44.8 ± 4.8 | 67.6 ± 7.5 | 69.2 ± 9.7 | 108.6 ± 9.0 | 28.7 ± 0.9 | 88.0 ± 3.9 | 53.8 ± 7.4 | 76.7 ± 5.4 | 36.2 ± 2.1 | 189.0 ± 27.8 |
| RR6 | 41.2 ± 2.7 | 16.0 ± 1.5 | 1,007.0 ± 71.5 | 102.9 ± 21.5 | 1,693.5 ± 105.6 | 217.6 ± 16.4 | 835.1 ± 121.0 | 4,691.6 ± 350.4 | 920.2 ± 65.5 | 441.4 ± 25.9 | 2,362.1 ± 95.5 |
| RR7 | 35.2 ± 10.5 | 4.7 ± 0.7 | 139.6 ± 18.0 | 37.1 ± 4.3 | 250.0 ± 39.7 | 62.0 ± 4.5 | 1,705.3 ± 254.7 | 4,993.6 ± 387.2 | 846.8 ± 25.7 | 1,693.5 ± 74.0 | 460.1 ± 28.6 |
| Other genes | |||||||||||
| cZOGT | 7.5 ± 1.5 | 29.3 ± 3.8 | 111.1 ± 18.0 | 341.5 ± 23.9 | 806.7 ± 54.2 | 153.9 ± 28.0 | 2,188.7 ± 63.4 | 817.9 ± 29.2 | 279.3 ± 21.4 | 258.8 ± 19.6 | 29.0 ± 2.4 |
| bGLU | 38.3 ± 4.3 | 3.1 ± 1.1 | 11.2 ± 1.5 | 523.1 ± 38.6 | 67.7 ± 4.8 | 2,531.6 ± 89.4 | 150,129 ± 14,771 | 32,448 ± 2,110 | 8,512 ± 1,324 | 11,081 ± 865 | 10.8 ± 2.5 |
| GALS | 54.8 ± 9.7 | <1.0 | 213.1 ± 14.5 | 5,134.0 ± 196.7 | 6,543.6 ± 266.6 | 126.7 ± 9.4 | 28.5 ± 5.8 | 134.0 ± 11.8 | 2,250.2 ± 92.7 | 3,605.2 ± 178.6 | 1,3087.2 ± 489.2 |
| VP14 | 143.6 ± 14.7 | 12.0 ± 2.7 | 605.0 ± 47.8 | 218.4 ± 38.0 | 926.6 ± 72.4 | 568.4 ± 28.4 | 35.3 ± 2.7 | 31.5 ± 5.1 | 607.1 ± 57.1 | 41.1 ± 4.4 | 664.4 ± 29.7 |