Table II.
Effects of the rst1 mutation on Arabidopsis leaf cutin monomers
The amount (μg dm−2) of total cutin monomers was higher in rosette leaves of the two isogenic allelic mutants rst1-2 and rst1-3 than in wild-type Col-0, due primarily to an increase in C18:2 dicarboxylic acids. Values represent means ± sd (n = 5). Asterisks indicate significant differences from the wild-type Col-0 amount as determined by Student's t test (P < 0.05).
| Cutin Monomersa | Col-0 | rst1-2 | rst1-3 |
|---|---|---|---|
| 16-OH C16:0 | 1.3 ± 0.4 | 1.8 ± 0.1 | 1.3 ± 0.2 |
| 10(9),16-OH C16:0 | 1.9 ± 0.8 | 2.3 ± 0.2 | 2.1 ± 1.2 |
| C16:0 dioic acid | 6.6 ± 0.6 | 7.2 ± 0.8 | 8.1 ± 0.7 |
| 18-OH C18:2 | 5.2 ± 0.9 | 6.0 ± 1.0 | 7.1 ± 1.7 |
| 18-OH C18:1 | 2.7 ± 0.6 | 2.8 ± 0.7 | 2.9 ± 0.2 |
| 18-OH C18:0 | 1.2 ± 0.3 | 1.3 ± 0.0 | 1.0 ± 0.1 |
| C18:2 dioic acid | 47.5 ± 4.5 | 56.2 ± 0.2* | 66.3 ± 7.7* |
| C18:1 dioic acid | 5.2 ± 0.6 | 5.6 ± 1.3 | 5.9 ± 0.3 |
| C18:0 dioic acid | 1.4 ± 0.2 | 1.6 ± 0.1 | 1.7 ± 0.2 |
| Total | 73.0 ± 6.0 | 84.8 ± 3.8* | 96.4 ± 8.0* |
16-OH C16:0, 16-Hydroxy hexadecanoic acid; 10(9),16-OH C16:0, 10(9),16-dihydroxy hexadecanoic acid; C16:0 dioic acid, hexadecane-1,16-dioic acid; 18-OH C18:0, 18-hydroxy octadecanoic acid; 18-OH C18:1, 18-hydroxy octadec-9-enoic acid; 18-OH C18:2, 18-hydroxy octadeca-9,12-dienoic acid; C18:0 dioic acid, octadecane-1,18-dioic acid; C18:1 dioic acid, octadecene-1,18-dioic acid; C18:2 dioic acid, octadecadien-1,18-dioic acid. OH denotes a hydroxyl functional group.