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Effect of temperature on TIR2 expression. GUS staining in 4-d-old seedlings in pTIR2:TIR2-GUS, pTIR2:GUS, and DR5:GUS lines at 22°C (4 d at 22°C) and at 29°C (2 d at 22°C, 2 d at 29°C). A, B, G, and H, pTIR2:TIR2-GUS. C, D, I, and J, pTIR2:GUS. E, F, K, and L, DR5:GUS. A, C, E, G, I, and K, 22°C. B, D, F, H, J, and L, 29°C. Bars = 1 mm in A to F, 250 μm in G to J, and 125 μm in K and L.
