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Phenotype of the tir2-1 tir7-1 double mutants. A, Root length of 7-d-old seedlings. B, Eight-day-old tir2-1 tir7-1 seedlings. C to E, Expression of QC25 (blue) and Lugol staining (dark blue) in Col-0 (C) and tir2-1 tir7-1 (D and E) seedlings. Bar = 50 μm for the 8-d-old seedlings (C–E). F to J, Expression of DR5rev:GFP (green) in Col-0 (F), tir2-1 (G), tir7-1 (H), and tir2-1 tir7-1 (I and J). Propidium iodide staining (red). Bar = 50 μm for the 5-d-old seedlings (F–J).
