Figure 2.

Developmental phenotypes of bob1 mutant plants. bob1-3 mutant plants exhibit pleiotropic developmental phenotypes. Col-0 plants have relatively smooth leaf margins with small serrations (A) compared to bob1-3 (B) or bob1-3;bob1-1 (C) mutant plants. bob1-3 flowers have more sepals, petals, and carpels and fewer stamens than Col-0 flowers. This floral organ phenotype is rescued by a BOB1:GFP transgene (D; n = 33, Student's t test P values versus Col-0 are <0.015* and <4 × 10⁻⁷**). Col-0 plants typically have four petals (E), while many bob1-3 flowers have five or more petals (arrowheads, F). Fused stamens were never observed in Col-O plants but are found in 30% of bob1-3 plants (inset, F). Overall plant architecture is changed in bob1 TH plants, which are short and branched. Many of these branches terminate after producing only a small number of flowers (box, G). Terminated branches in this background are due to pin-formed meristems (H). Col-0 (I) and bob1-3 (J) anthers produce pollen, while bob1-3/bob1-1 TH anthers do not (K). TH anthers have patches of stigmatic papillae (arrowheads in K, close-up in L). Bars = 1 cm in A to C and G, 250 μm in H, and 50 μm in I to L.