Figure 5.

Thermotolerance of bob1-3 and Col-0 plants. Col-0 and bob1-3 seeds were stratified for 2 d and then exposed to a 1-h heat shock before plating. Seedling germination was scored 7 d after plating (A). The thermotolerance defect with a 46.4°C heat shock during germination can be rescued by the introduction of a BOB1:GFP transgene (B). Survival of 3-d-old Col-0, bob1-3, and hot1-3 seedlings after a 60- or 90-min 44°C heat shock with or without a 15-min 37°C acclimation period was observed 7 d after the heat shock (C). Error bars in A represent 99% binomial confidence intervals.