Figure 1.

Effects of various environmental stresses on thiamin content. A, Thiamin levels of 9-d-old whole Arabidopsis plants grown under heat (24 h at 38°C), osmotic (50 mm sorbitol), cold (24 h at 4°C), oxidative (0.25 μm paraquat), and salt (50 mm NaCl) stresses analyzed using HPLC. B, Free thiamin, TMP, and TPP levels of 9-d-old whole plants exposed to paraquat-induced oxidative stress. All plants were grown in 100 μmol m⁻² s⁻¹ constant light. Values represent means ± sd of three to five independent experiments. Student's t tests were conducted to compare the thiamin content/composition of stressed plants with control plants. **, Student's t test significant at P < 0.01; *, Student's t test significant at P < 0.05.