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Quantitative RT-PCR of OPR expression in response to TNT treatment. Fourteen-day-old, liquid culture-grown Arabidopsis plants were treated with 60 μm TNT for 6 h, and the level of OPR expression was determined by quantitative RT-PCR. ACT2 mRNA was used to normalize cDNA levels. Results are means of three independent RNA isolations measured in triplicate ± se.
