Figure 2.

Homoplasmy and phenotypes of transplastomic tomato lines. A, Examples of seed tests to confirm homoplasmy. Seeds from the wild-type (S.l.-wt) and transplastomic plants generated with constructs pEcrtY and pNLyc were germinated on medium with spectinomycin (100 mg/L). Antibiotic resistance and lack of segregation in the T1 generation confirms the homoplasmic state of the transplastomic lines. B, Wild-type-like phenotype of homoplasmic tomato lines expressing lycopene β-cyclase transgenes from their plastid genomes.