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Western-blot analysis of proteins from thylakoid membrane fractions isolated upon sonication and differential centrifugation at 10,000g (10k), 40,000g (40k), and 140,000g (140k). Lanes were loaded with 2 μg of chlorophyll (Chl). Proteins were probed with specific polyclonal antibodies generated against D1 (A) and REP27 (B). C shows a Coomassie Brilliant Blue-stained SDS-PAGE protein profile of the different thylakoid membrane fractions.
