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. 2009 Sep;151(1):142–154. doi: 10.1104/pp.109.143404

Figure 1.

Figure 1.

WPP domain proteins WPP1 and WPP2 interact with both WIT1 and HSC70 in vivo. A, HA-HSC70-1 was coexpressed with either GFP, WPP1-GFP, or WPP2-GFP (left); HA-HSC70-3 was coexpressed with either GFP, WPP1-GFP, or WPP2-GFP (right) in N. benthamiana. Immunoprecipitation was performed using the anti-HA antibody, and coimmunoprecipitated proteins were detected with the anti-GFP antibody. p19 was present in all N. benthamiana infiltrations. B, HA-WPP1 or HA-WPP2 was coexpressed with either GFP or GFP-WIT1 in N. benthamiana. Immunoprecipitation was performed using the anti-HA antibody, and coimmunoprecipitated proteins were detected with the anti-GFP antibody. p19 was present in all N. benthamiana infiltrations. C, Samples immunoprecipitated from 35S:GFP, WPP1:WPP1-GFP, and WPP2:WPP2-GFP transgenic lines using the anti-GFP antibody (top) were probed with the anti-WIT1 (middle) or the anti-Hsc70 antibody (bottom). IP, Immunoprecipitate.