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. 2009 Sep;151(1):142–154. doi: 10.1104/pp.109.143404

Figure 4.

Figure 4.

The three-amino-acid WPP motif of WPP1 is required to prevent the GFP-WIT1 aggregates. A, GFP-WIT1 was coexpressed with either HA-WPP1 or HA-WPP1WPP/AAP in N. benthamiana leaves by coagroinfiltration in the presence of p19. A, Aggregates. Bars = 10 μm. B, WPP1WPP/AAP has reduced affinity for WIT1. GFP-WIT1 was coexpressed with either HA-WPP1 or HA-WPP1WPP/AAP in N. benthamiana leaves by coagroinfiltration in the presence of p19. Immunoprecipitation was performed using the anti-HA antibody, and coimmunoprecipitated protein was detected with the anti-GFP antibody. The positions of proteins are depicted with arrows. IP, Immunoprecipitate. [See online article for color version of this figure.]