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Expression analyses of AtPAP15 in the three independent transgenic soybean plants. A, Southern-blot analysis of transgenic lines using the Bar probe. DNA was digested by EcoRI or HindIII. M, Marker; WT, wild-type soybean; 1-8, 2-3, and 3-4, plants transformed with AtPAP15. B, Detection of AtPAP15 protein in the roots of transgenic lines by western-blot analysis. WT, Wild-type soybean; 1-8, 2-3, and 3-4, plants transformed with AtPAP15.
