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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Hear Res. 2005 Jun;204(1-2):156–169. doi: 10.1016/j.heares.2005.02.002

Figure 5. Saturability of cytoplasmic, but not vesicular GTTR fluorescence.

Figure 5

OK or MDCK cells were treated with 1 μg/mL GTTR for 120 minutes at 37°C or held over ice in the presence of increasing concentrations of unconjugated gentamicin. Cells were imaged live or fixed with FATX and washed before imaging. A1-A5) At 37°C, OK cells, treated with GTTR show serially reduced labeling with increasing unconjugated gentamicin (GT) concentrations. Inset in A1) shows only vesicular GTTR labeling in live OK cells treated with GTTR alone. Inset in A5) also shows little or no decrease in vesicular GTTR labeling in live OK cells treated with GTTR plus 4 mg/mL unconjugated gentamicin. B1-B5) OK cells on ice show reduced GTTR binding in the cytoplasm and intra-nuclear compartments (compared to 37°C, A1-A5) and reduction of binding with increasing concentrations of unconjugated gentamicin concentrations. Inset in B1) shows no vesicular GTTR uptake in OK cells on ice when imaged live. C1-C5) MDCK cells on ice also show decreasing cytoplasmic GTTR fluorescence as concentration of unconjugated gentamicin increases.