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ARH reproduction-regulating neuropeptide mRNA expression analyzed by real-time PCR for Kiss1, NKB, and PDYN in experiments 1–3. Con, Virgin control; Lac, lactation + eight pups; Lac + Ins, − lactation + insulin replacement; Lac + Lep; lactation + leptin replacement; Lac + Ins/Lep, lactation + insulin and leptin replacement; − Pups, 48 h pup removal. Columns with different letters are significantly different (P < 0.05).
