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. 2009 Sep 1;106(37):15645–15650. doi: 10.1073/pnas.0904984106

Fig. 3.

Fig. 3.

D-elp1 is required for RNAi and interacts with Dcr-2. (A) Quantitative RT-PCR analysis for the expression levels of Dcr-2, D-elp1, D-elp2, and D-elp3 RNAs in S2 cells after depletion. (B) S2 cells were soaked with dsRNA either to GFP, Dcr-2, D-elp2, D-elp3, and D-elp1 (a mixture of D-elp1.A and D-elp1.B) and were then cotransfected with Renilla and firefly luciferase reporters, with or without dsRNA to firefly luciferase, to determine changes in firefly luciferase activity due to the inhibition of RNAi. Three separate experiments are shown. (C) D-elp1 interacts with V5 tagged Dcr-2 in S2 cells. Western blot analysis was performed using either antiFlag or V5 antibody in the initial pulldown followed by blot analysis with the counter antibody. The Ig heavy and light chains are indicated by IgG-hc and IgG-lc, respectively. (D) Ectopically expressed D-elp1 and either Ago-2 or Dcr-1 show weak but reproducible interactions in S2 cells.