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. Author manuscript; available in PMC: 2010 Aug 28.
Published in final edited form as: Mol Cell. 2009 Aug 28;35(4):442–453. doi: 10.1016/j.molcel.2009.06.030

Figure 7. Inverse correlation between Chk1 and Fbx6 expression.

Figure 7

Asynchronously growing cells derived from (A) various tumor types and (B) non-small cell lung cancer were lysed and blotted with anti-Chk1 and anti-Fbx6 antibodies, and the same membranes were stripped and re-blotted with anti-tubulin and anti-PCNA antibodies, respectively. (C) Representative staining of Chk1 and Fbx6 in breast tumor sections. (D) The breast tumor staining data were analyzed and quantitated as described in Experimental Procedures. (E) Model for Chk1-regulated replication checkpoint activation and termination. The distal C-terminus of Chk1 interacts with the kinase domain likely through adaptor proteins (marked with ‘X’). After the phosphorylation-induced conformational change, the CM (conserved motif) and part of the regulatory domain form the degron-like region of Chk1. For simplicity, the spatial regulation of Chk1 is omitted in this model. See text for details.