Skip to main content
. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Curr Biol. 2008 Jun 19;18(13):943–950. doi: 10.1016/j.cub.2008.06.020

Figure 3. let-7 Misexpression Causes Premature Cell-Cycle Exit in the Wing.

Figure 3

(A) Cell number in “flp-out” clones of cells misexpressing UAS-let-7 and UAS-GFP; control clones express UAS-GFP alone (see Experimental Procedures). Most let-7-expressing cells stop dividing after 30 hr of clonal growth. Controls: n = 52 at 20 hr, n = 41 at 30 hr, and n = 34 at 40 hr; UAS-let-7: n = 34 at 20 hr, n = 31 at 30 hr, and n = 31 at 40 hr. Error bars represent standard error.

(B and C) The wing-specific driver, Vestigial (Vg) Gal4, was used to misexpress UAS-GFP alone (control), or in combination with UAS-let-7 or UAS-miR-125 in larval wing discs. UAS-let-7 expression significantly reduced disc size, whereas UAS-miR-125 had no effect. Numbers in each bar represent discs measured. (C) Misexpression of UAS-let-7 under Vg Gal4 control significantly reduces adult wing size compared to controls that express UAS-GFP alone (p < 0.001; control: n = 50; UAS-let-7: n = 50). In contrast, misexpression of UAS-miR-125 does not alter wing size (p > 0.05; control: n = 20; UAS-miR-125: n = 20). Boxes represent second- and third-quartile values; whiskers extend to the maximum and minimum values. Inset shows the size difference of overlaid UAS-let-7-misexpressing (blue) and control (gray) wings.