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Tracing rhodopsin diffusion at physiologic pH in the presence of hydroxylamine. Oblique arrow in A points to initial absorbance increase at the bleached side due to the formation of extra Meta I-like product in hydroxylamine-containing solution. Multiphase absorbance difference changes seen up to 30 s postbleach in B were due to formation of metaproducts and their subsequent conversion to retinaloxime. ROS diameter is 11.6 μ, the apparent diffusion constant Da=1.3×10−9 cm2s−1.
