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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Mol Carcinog. 2009 Sep;48(9):801–809. doi: 10.1002/mc.20524

FIGURE 3.

FIGURE 3

Stimulation of migration by lysophosphatidylcholine depends on autotaxin activity. Panel A shows the migration of MDA-MB-231 cells over 3 h in the presence of basal medium or concentrated medium prepared from either MDA-MB-231 cells (231 cMedium), or from MDA-MB-435 cells (435 cMedium). These experiments were performed in the presence or absence of 10 μM LPC, or 1 μM of the ATX inhibitor, VPC8a202. Migration of MDA-MB-231 cells with only basal media (RPMI 1640 with 0.1% BSA and 0.2% FBS) present was given a value of 1. Migration in the presence of 5% FBS and 0.5 μM LPA plus VPC8a202 were used as controls. Panel B, shows results from similar experiments using MDA-MB-435 cells over a 3 h period. Panel C shows the effects of the ATX inhibitors, VPC8a202 and S32826, on the migration of MDA-MB-435 cells over 3 h in the absence of concentrated medium. Results are means ± SEM from at least 3 independent experiments. Statistically significant differences are indicated by: * (p < 0.01).