Table 1.
Tm values for cycles 1–3 in the presence of DNA.
| dsDNA sequence = | 5′–TTGC TGTTCT GCAA–3′ 3′–AACG ACAAGA CGTT–5′ |
|
|---|---|---|
| polyamide cycle | Tm/°C | ΔTm/°C |
| – | 60.0 (±0.3) | – |
 (1) |
83.5 (±0.5) | 23.6 (±0.6) |
 (2) |
60.1 (±0.6) | 0.1 (±0.6) |
 (3) |
83.0 (±0.3) | 23.1 (±0.4) |
All values reported are derived from at least three melting temperature experiments with standard deviations indicated in parentheses. ΔTm values are given as Tm(DNA/polyamide) - Tm(DNA). The propagated error in ΔTm measurements is the square root of the sum of the square of the standard deviations for the Tm values.