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. Author manuscript; available in PMC: 2010 Mar 31.
Published in final edited form as: Biochemistry. 2009 Mar 31;48(12):2630–2642. doi: 10.1021/bi801853a

Figure 5.

Figure 5

Surface plasmon resonance analysis of the binding of Gαi1 subunits to nucleotide-state selective peptides. Surface plasmon resonance demonstrates peptides KB-752 and KB-1753 discriminate between activation state of Gαi1 proteins. In each graph, the dashed line represents binding of the indicated Gα subunit to immobilized peptide KB-752, selective for inactive (GDP-bound) Gα subunits, while the solid line represents binding to immobilized peptide KB-1753, selective for activated (GTP-bound) Gα subunit. (a) Wild-type Gαi1in the presence of GDP; (b) Wild-type Gαi1 in the presence of GDP-AlF4−; (c) I56C/Q333C Gαi1 in the presence of GDP; (d) I56C/Q333C Gαi1 in the presence of GDP-AlF4−. (e) D328R Gαi1 in the presence of GDP; (f) D328R Gαi1 in the presence of GDP-AlF4−.