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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Gastroenterology. 2009 Mar 26;137(3):1051–1060.e3. doi: 10.1053/j.gastro.2009.03.040

Figure 3.

Figure 3

Myosin II is associated with RNAP II, TFIIB, α-actin and β-actin in the nucleus. Nuclear extracts were immunoprecipitated (IP) with indicated antibodies, followed by immunoblotting (IB). Rabbit IgG was used as negative control. (A) RNAP II co-precipitated with MHC and MLC20. MHC co-precipitated MLC20. (B) TFIIB antibody pulled down MHC and MLC20 and MLC20 pulled down MHC. (C) β-actin co-precipitated with MLC20. (D) Anti-MLC20 antibody precipitated both α-actin and β-actin.