Figure 6.

Neutralizing activity of rabbit immune sera, anti-V3 mAb pools, and a broadly neutralizing HIV+ serum against pseudoviruses expressing the Envs of patient-derived isolates. Twenty pseudoviruses carrying the Envs from clade A, B, C or D viruses were tested against immune sera from rabbits immunized with gp120 DNA from clade C and V3-FPs, as per Table 1. The percent neutralization displayed by rabbit immune sera diluted 1:10 is shown against the 20 psVs as well as the negative control aMLV psV. While analysis of pre-bleed sera indicates that neutralization of ≥40% is statistically significant (p>0.05), the more conservative convention is used in this figure where only serum/psV combinations achieving 50% neutralization are denoted by black squares. The same viruses were tested for neutralization by four anti-V3 mAb pools under conditions in which the final concentration of each pool of four mAbs was 50 ug mAbs/ml. The average percent neutralization by the four pools is shown, and viruses are listed, left to right, in terms of most to least sensitive to anti-V3 mAb neutralization. Positive neutralization (defined in the text) is demarcated by gray and black boxes. The same viruses were also tested for neutralization sensitivity to an HIV+ serum, Z23. The 50% reciprocal neutralization titers (NT₅₀) against each pseudovirus are shown. Positive neutralization (defined in the text) is demarcated by gray and black boxes.