. Author manuscript; available in PMC: 2009 Sep 15.

Published in final edited form as: Virology. 2009 Jul 26;392(1):82–93. doi: 10.1016/j.virol.2009.05.039

Table 1.

Immunization Regimen^*

Immunizing Regimen	DNA prime^† at weeks 0, 2, 4	Protein boost^† at weeks 10 & 14
-/ABC	- -	V3_A-, V3_B-, & V3_C-FP
A_R/ABC	gp120/Clade A (GPGR)	V3_A-, V3_B-, & V3_C-FP
C_Q/ABC	gp120/Clade C (GPGQ)	V3_A-, V3_B- & V3_C-FP
A_{R +} C_Q/ABC	gp120/Clade A (GPGR) & gp120/Clade C (GPGQ)	V3_A-, V3_B- & V3_C-FP

C_Q/A	gp120/Clade C (GPGQ)	V3_A-FP
C_Q/B	gp120/Clade C (GPGQ)	V3_B-FP
C_Q/C	gp120/Clade C (GPGQ)	V3_C-FP
C_Q/AC	gp120/Clade C (GPGQ)	V3_A- & V3_C-FP

Two immunization experiments were performed. The groups shown above and below the dotted line were included in each of the experiments, respectively.

^†

V3 sequences contained in priming and boosting constructs are shown below. V3-FPs used for protein boosts were based on various V3 sequences fused to gp70 of MuLV. Variations in sequence from relevant consensus sequences are underlined, and the variation at the tip of the loop, position 18 (R/Q), is bolded.

CA1 clade A1 env gp120 DNA prime (A_R):	CTRPNNNTRKGIHIGPGRAIYATGDIIGDIRQAHC
92BR025.9 clade C env gp120 DNA prime (C_Q):	CTRPNNNTRKSIRIGPGQAFYATGEIIGDIRQAHC
V3_A-FP derived from clade A strain 92UG037.08:	CTRPNNNTRKSVRIGPGQTFYATGDIIGDIRQAHC
V3_B–FP derived from clade B strain JR-CSF:	CTRPSNNTRKSIHIGPGRAFYTTGEIIGDIRQAHC
V3_C-FP derived from clade C strain 93IN904:	CTRPNNNTRKSIRIGPGQTFYATGDIIGDIRQAHC