Table 2. Summary of next generation sequencing results.
Strain | NM20/02 | NM31/04 | Jp8756 | |
Sequencing method | Roche 454 GS FLX | Illumina Solexa GA | Illumina Solexa GA | |
Total no. Reads | 424,494 | 2,538,429 | 2,651,276 | |
Averaged read length (nt) | 213 | 35 | 35 | |
Total sequences (nt) | 90,299,836 | 88,845,015 | 92,794,660 | |
Map to Agy99 chromosome | Total no. reads mapped (%) | 382,116 (90.01) | 2,343,269 (92.31) | 2,279,741 (85.99) |
% genome mapped | 93.72 | 99.99 | 94.47 | |
Average depth of mapped regions | 14.5 | 14.1 | 13.6 | |
Map to Agy99 plasmid pMUM001 | Total no. reads mapped (%) | 5,269 (1.24) | 326,541 (12.86) | 89,325 (3.37) |
% genome mapped | 32.56 | 100 | 20.35 | |
Average depth of mapped regions | 19.8 | 63.4 | 17.2 |