| pRS316 |
pBluescript-based centromeric yeast expression vector with URA3 gene for selection |
ATCC |
| pRS316-Sc_HSP104
|
pRS316 expressing the HSP104 gene from S. cerevisiae under the control of the endogenous promoter (588 bp) |
S. Lindquist Lab |
| pRS316-Sp_hsp104+
|
pRS316 expressing the hsp104+ gene from S. pombe under the control of the endogenous S. cerevisiae HSP104 promoter (588 bp) |
This study |
| pRS315 |
pBluescript-based centromeric yeast expression vector with LEU2 gene for selection |
ATCC |
| pRS315-Sp_hsp104+
|
pRS315 expressing the hsp104+ gene from S. pombe under the control of the endogenous S. cerevisiae HSP104 promoter (588 bp) |
This study |
| pGPD-luxAB(HIS) |
p426GPD vector expressing a bacterial temperature-sensitive Vibrio harveyi luciferase |
S. Lindquist Lab |
| pYSGAL |
pRS316 with the galactose-inducible GAL1 overexpression promoter |
S. Lindquist Lab |
| pYSGAL-Sc_HSP104
|
pYSGAL overexpressing the HSP104 gene from S. cerevisiae
|
S. Lindquist Lab |
| pYSGAL-Sp_hsp104+
|
pYSGAL overexpressing the hsp104+ gene from S. pombe
|
This study |
| pYSGAL-Sc_HSP104/CTDSp
|
pYSGAL overexpressing a chimeric HSP104 gene from S. cerevisiae (first 2190 bp) with the CTD of the hsp104
+ gene from S. pombe (last 507 bp) |
This study |
| pYSGAL-Sp_hsp104/CTDSc
|
pYSGAL overexpressing a chimeric hsp104
+ gene from S. pombe (first 2211 bp) with the CTD of the HSP104 gene from S. cerevisiae (last 537 bp) |
This study |
