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. 2009 Sep 14;4(9):e6943. doi: 10.1371/journal.pone.0006943

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Florins et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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CFSE was injected intravenously in BLV infected sheep and in non infected controls. At regular interval of time, the percentage of B lymphocytes labeled with CFSE was determined by flow cytometry. A mathematical model was fitted to in vivo CFSE labeling data in B lymphocytes and death rates were estimated. Not splenectomized (left panels) and splenectomized (right panels) animals were analyzed. (A) Percentages of B cells labeled with CFSE in BLV-infected (▪) and control (▴) sheep. (B) Mean values (±SD) of death rates. CFSE was injected in 7)infected sheep, 3 of them being splenectomized and 7 controls, 3 of them being splenectomized. Statistical relevance (n.s., not significant; p<0.05, statistically significant*; p<0.01, highly statistically significant**) was calculated according to the unpaired two-tailed student t test with Welch's correction.