Abstract
The growth of Streptococcus pneumoniae in commercial media containing 14C-labeled substrates was studied experimentally; the results of blood cultures that were positive for S. pneumoniae over a 14-month period were analyzed to explain no-growth but radiometrically positive blood cultures from four patients with clinically diagnosed pneumococcal pneumonia. The growth of S. pneumonoiae in aerobic blood culture vials resulted in a chocolate color in the medium. S. pneumoniae grew rapidly in both aerobic and anaerobic media, but 14CO2 evolved from the metabolism of the labeled substrates was detected only in the aerobic culture vials. Radiometric detection lagged behind growth of the organisms and was accompanied by visual changes in the media. By 24 h, the viability of the culture was on the decline; radiometric readings remained positive even when the culture had died.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bannatyne R. M., Harnett N. Radiometric detection of bacteremia in neonates. Appl Microbiol. 1974 Jun;27(6):1067–1069. doi: 10.1128/am.27.6.1067-1069.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gröschel D., Hopfer R. L., French J. E. Blood cultures with the BACTEC 225 radiometric microbial growth detection system. Zentralbl Bakteriol Orig A. 1979 Jul;244(2-3):316–323. [PubMed] [Google Scholar]
- Lorian V., Popoola B. Pneumococci producing beta hemolysis on agar. Appl Microbiol. 1972 Jul;24(1):44–47. doi: 10.1128/am.24.1.44-47.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thiemke W. A., Wicher K. Laboratory experience with a radiometric method for detecting bacteremia. J Clin Microbiol. 1975 Mar;1(3):302–308. doi: 10.1128/jcm.1.3.302-308.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Washington J. A., 2nd, Yu P. K. Radiometric method for detection of bacteremia. Appl Microbiol. 1971 Jul;22(1):100–101. doi: 10.1128/am.22.1.100-101.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Waters J. R. Sensitivity of the 14 CO 2 radiometric method for bacterial detection. Appl Microbiol. 1972 Jan;23(1):198–199. doi: 10.1128/am.23.1.198-199.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Williams G. T., Houang E. T., Shaw E. J., Tabaqchali S. Bacteraemia in a London teaching hospital 1966-75. Lancet. 1976 Dec 11;2(7998):1291–1293. doi: 10.1016/s0140-6736(76)92044-4. [DOI] [PubMed] [Google Scholar]