Fig. 5.

E. coli expulsion by BECs requires MyRIP activity. (A) A pull-down assay was performed using Rab27-, MyRIP-, and PKA RII-specific antibodies. A control immunoblot revealed that equal levels of caveolin-1 protein were expressed in the cell extracts before and after infection (Inset). All these signaling molecules were coassociated with caveolin-1 in BECs even before infection (UI) and after infection (EC), the coassociation became markedly more increased. (B) An E. coli exocytosis assay was performed to examine effects of MyRIP on bacteria expulsion. MyRIP mRNA levels were reduced by ≈40% in kd BECs (Inset), and levels of E. coli exocytosis were significantly dropped by 40%. *, P < 0.002 by unpaired t test when compared with control siRNA transfected BECs. Error bars represent SEM.