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. 2009 Aug 19;106(35):14831–14836. doi: 10.1073/pnas.0900742106

Fig. 4.

Fig. 4.

The knockdown of ERFAD decreases the cellular amount of polyubiquitinated proteins. (A) Accumulation of polyubiquitinated proteins upon MG132 treatment. HEK293 cells were either left untreated or treated with MG132, lysates were adjusted by a BCA assay to the same protein concentration and analyzed by Western blotting with anti-ubiquitin. (B) Decrease of polyubiquitinated proteins upon ERFAD knockdown. HEK293 cells were transfected with ERFAD siRNA#1 and nonsilencing control siRNA (c). Cells were lysed 72 h after transfection, lysates were adjusted to the same concentration and analyzed by Western blotting with anti-ubiquitin and anti-actin. (C) Quantification of three independent experiments performed as described in B and plotted as percentage of control, mean ± SD.