Secondary structure dynamics of Aβ assemblies. Uncross-linked (UnXL), cross-linked (XL), or fibrillar (Fib) Aβ (A) or isolated oligomers [monomer (Mo), dimer (Di), trimer (Tr), and tetramer (Te)] (B) were prepared in 10 mM PBS, pH 7.4, and then monitored immediately by CD. Data are representative of three independent experiments.