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. 2009 Aug 18;106(35):14808–14813. doi: 10.1073/pnas.0907799106

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Fig. 4. — MTeC reveals that full-length G9a selectively binds H3K9me1 in vivo. (A) Silver stain of FLAG-purified H3-G9a mutant and H3K9R-G9a mutant control MTeC bait fusion proteins (asterisk) from HEK 293 nuclear lysates. MS identified ≈70-kDa band as G9a (arrow). (B) Western blot analysis of FLAG-purified H3-G9a MTeC bait fusion proteins from COS7 nuclear lysates indicates a distinct H3K9 methylation pattern compared with HEK 293 cells (Fig. 2B). (C) Western blot analysis of HA-purified COS7 nuclear lysates cotransfected with the indicated FLAG-tagged H3-G9a MTeC bait plasmids and either an HA-tagged G9a ankyrin repeats only or full-length HA-G9a plasmid (D).