Fig. 1.
Vj-gating and modulation of electrical signaling asymmetry. (A) Fluorescence image of a HeLaCx43-EGFP/HeLaCx45-CFP cell pair exhibiting a heterotypic junctional plaque (JP; see Inset). An internalized heterotypic JP is shown by the arrow. (B) gj-Vj dependence measured in the cell pair shown in A by applying slow (1 mV/s) Vj ramps, demonstrates voltage-gating asymmetry. (C) Modulation of intercellular electric signaling asymmetry in the cell pair shown in A. V1 and V2 traces show the voltage protocol applied to HeLaCx45-CFP (cell-1) and the electrotonic potentials measured in HeLaCx43-EGFP (cell-2), respectively. Red arrows indicate a 12 mV increase of the holding potential in cell-1 starting from −12 mV and going to 0 mV and then to 12 mV (Bottom Insets 1, 2 and 3, respectively).