Fig. 5.

Summarized data of J_j-V_j for AF³⁵⁰ measurements in 25 Cx43/Cx45 cell pairs. Data were normalized to J_j at V_j = 0 mV. Red (synergistic) and gray (antagonistic) circles indicate experimental data in which V_j steps of negative or positive polarity were applied to either cell of the cell pair. Red and gray curves show fitting of the data encompassed in the yellow square and shown in red and gray circles, respectively, by a sigmoidal equation. Green (synergistic) and blue (antagonistic) filled circles indicate experimental data in which high frequency bursts of pulses of positive polarity were applied to either cell of the cell pair (V_j was positive when the Cx43 cell was stimulated and negative when the Cx45 cell was stimulated, see Fig. 6). The data presented show that J_j dependence on V_j in experiments with synergistic action of ionophoresis and V_j-gating (red and green filled circles) is steeper around V_j = 0 mV than in experiments with antagonistic action of ionophoresis and V_j-gating (gray and blue filled circles). The black line shows normalized g_j-V_j plot, averaged from five experiments.