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Purification of Arabidopsis TPST
| Purification step | Total protein, mg | Total activity, units | Specific activity, units/mg | Yield, % | Purification, fold |
|---|---|---|---|---|---|
| MM2d microsomal membranes | 1,500 | 25,000 | 16.7 | 100 | 1.0 |
| TX-100 solubilization | 1,200 | 22,500 | 18.8 | 90 | 1.1 |
| pPSY1[43–57]-Sepharose column | <0.1 | 3,750 | >37,500 | 15 | >2,250 |
| Hydroxyapatite column | 0.018 | 2,750 | 153,000 | 11 | 9,170 |
