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. 2009 Aug 24;106(35):14914–14919. doi: 10.1073/pnas.0902882106

Fig. 5.

Fig. 5.

HSF-1 or DAF-16 modulate the age-dependent misfolding of multiple metastable proteins. (A) Age-synchronized (L4) animals expressing paramyosin(ts) [unc-15(e1402)] or dynamin(ts) [dyn-1(ky51)] were transferred to control (L4440), hsf-1, daf-16, or age-1 RNAi-expressing bacteria and scored for slow movement (black) or defective ssGFP uptake (gray) phenotypes respectively. Data are mean ± SD, >92 per RNAi treatment. (B) Age-synchronized (L4) animals expressing ras(ts) [let-60(ga89)] were transferred to control (circles), daf-16 (squares), or hsf-1 (triangles) RNAi-expressing bacteria and scored for percentage of Osm phenotype with age. Data are mean ± SD, >46 per data point. (C) Age-synchronized dynamin(ts) animals overexpressing hsf-1 (triangles) (hsf-1 o/e, strain AM586) or daf-16 (squares) (daf-16 o/e, strain AM707) were scored for percentage of animals showing dynamin mislocalization with age. Dashed line is dynamin(ts) animals for reference. Data are mean ± SD, >45 animals per data point. (D) Age-synchronized ras(ts) animals overexpressing hsf-1 (triangles) (hsf-1 o/e*, strain AM708) or daf-16 (squares) (daf-16 o/e*, strain AM558) were scored for percentage of animals showing Osm phenotype with age. Dashed line is ras(ts) for reference. Data are mean ± SD, >65 animals per data point. The roller phenotype (rol-6) did not affect the behavior scored (we observed 4.5 ± 4.8 compared to 8.9 ± 8.4% Osm phenotype on day 9 of adulthood of rol-6 or WT animals, respectively).