Fig. 3.
MMP14-mediated sorting requires signaling through ROCK. Frequency maps quantifying location of YFP-expressing cells co-transfected with MMP14 and treated with vehicle(A) or the ROCK inhibitor Y27632 (10 μM) (B). MMP14 sorting is phenocopied by ROCK, as shown in frequency maps of tubules mosaic for constitutively active ROCKΔ3 (C) or dominant negative ROCKKDIA (D). MMP14 activates ROCK signaling, as shown in E Western blots for phosphorylated LIMK (pLIMK) and total LIMK. (Scale bars, 50 μm.)