Fig. 3.

Antigen-specific TCR transgenic CD8⁺ T cells facilitate viral clearance through transfer of their antigen specificity to CD8⁺ T cells of unrelated specificity in vivo. Enriched splenic CD8⁺ T cells from IAV-immune B6.SJL mice (Fig. S1) and/or FACS purified Prf^−/− or WT OT-I cells that had been activated in vitro with SIINFEKL-pulsed splenic dendritic cells for 5 days (Fig. S2) were transferred into either B6.RAG-1^−/− or WT recipient mice 24 h after infection with 5 × 10⁴ PFU ECTV-OVA. (A and B) Splenic viral load (A) (*, P < 0.05; NS, not significant) and CTL activity (B) were measured 4 days after infection and 3 days after transfer of Prf^−/− OT-I and/or IAV-immune cells into RAG-1^−/− mice. (C) The cytotoxic activity of FACS sorted splenic CD8⁺ T lymphocytes, based on CD45 allotypes, was tested on SIINFEKL-pulsed targets 4 days after infection and 3 days after transfer of WT OT-I and/or IAV-immune cells to WT recipient mice.