(A) in vitro culture competition assay of wildtype CFT073 (filled symbols) and hma mutant (open symbols) cultured in Luria broth (solid line) or W salts minimal medium (dashed line). After inoculation (input CFU/ml plotted on y-axis), cultures were passaged into fresh medium every 8 (1:50 dilution) or 16 (1:500 dilution) hours. Arrows indicate culture passages. Means of triplicate cultures are plotted. *P=0.03.
(B) 72 hour CBA/J mouse co-infection with 108 CFU mixture of wildtype (WT) and hma mutant. Data points represent CFU/g of individual animals in the organs indicated; bars show median values (n=24).