Table 3.
Bacterial strains and plasmids.
| E. coli strain | Descriptiona | Reference or source |
|---|---|---|
| CFT073 | Pyelonephritis isolate | (Mobley et al., 1990) |
| K12 | MG1655, laboratory strain | (Blattner et al., 1997) |
| HB101 ent | HB101 ent::Tn5 strain 1017; Kanr | (Torres and Payne, 1997) |
| chuA | CFT073 chuA::cat; Camr | (Torres et al., 2001) |
| hma | CFT073 Δc2482::kan; Kanr | This study |
| hma chuA | CFT073 Δc2482::kan chuA::cat; Kanr, Camr | This study |
| tonB | MG1655 ΔtonB::kan, Kanr | This study |
|
| ||
| Plamid | ||
| pKD4 | λ Red template vector; Kanr Ampr | (Datsenko and Wanner, 2000) |
| pKD46 | Red recombinase helper plasmid, temp-sensitive; Ampr | (Datsenko and Wanner, 2000) |
| pGEN | pGEN-MCS, promoter-less expression vector, p15A ori (copy number ~15), par hok sok mok parM parR; Ampr | (Galen et al., 1999; Lane et al., 2007) |
| pnativehma | hma with native promoter (900 bp upstream) in pGEN-MCS | This study |
| pBAD-myc-HisA | Expression vector, pBR322 ori (low copy), araBAD promoter (arabinose-inducible), araC; Ampr | Commercial (Invitrogen) |
| phma | hma in pBAD | This study |
| pY126A | hmaY126A in pBAD | This study |
| pchuA | chuA in pBAD | This study |
| piutA | iutA in pBAD | This study |
| phma-His | hma in pBAD with C-term. His6 tag | This study |
| pchuA-His | chuA in pBAD with C-term. His6 tag | This study |
| piutA-His | iutA in pBAD with C-term. His6 tag | This study |
a
Kan, kanamycin; Cam, cholamphenicol; Amp, ampicillin