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Site-directed mutagenesis primer sequencesa.
| Mutation | Forward | Reverse |
|---|---|---|
| H242A | GGTTATAACTCCGGAAACGCTCGTTTTGGCCTCTCGC | GCGAGAGGCCAAAACGAGCGTTTCCGGAGTTATAACC |
| H331A | CAGGCTCTGACCGTTGCTAACAAGACTGACACCCATG | CATGGGTGTCAGTCTTGTTAGCAACGGTCAGAGCCTG |
| H337A | CATAACAAGACTGACACCGCTGATAAGCAATACACTC | GAGTGTATTGCTTATCAGCGGTGTCAGTCTTGTTATG |
| Y126A | GCGCGCCGGAGATAATGCTGGTGTGGGACTGTTG | CAACAGTCCCACACCAGCATTATCTCCGGCGCGC |
all sequences listed 5’→ 3’
