Table 2. PCR primers used in this study.
| Primer | Sequence (5′ → 3′) | Gene target |
NCBI accession number |
Target sequence position |
Product length |
|---|---|---|---|---|---|
| bp | |||||
| F1 | ATGACCGCTTCTGATACAGTCTAC | arz1 | NC_003421.2 | 2,325,172-2,325,196 | 2479 |
| R1 | TTCGCAACGCTGGAAAGTTTGTGT | 2,327,651-2,327,627 | |||
| F2 | TTTCAATCTCATTCTCACTTTCTGA | nmt/arz1 | pREP (23) | 1,098-1,122 | 374 |
| R2 | GGGCTCGATACTCTCGTGAATCC | NM_001023516.1 | 325-303 | ||
| F3 | CTCTTGAACTCCTTAGGG | arz1 | NM_001023516.1 | 98-115 | 665 |
| R3 | TGCTATTAGAGTCGTCGG | 763-746 | |||
| F4 | TCCCTTCTTCAGTCATGCCC | zfs1 | NM_001022372.1 | 251-270 | 460 |
| R4 | ACAGAGTTGCGGATGTTTGTC | 711-690 | |||
| F5 | TTGTTGACTGAGGCTCCTTTGAAC | actin | NM_001021513.1 | 310-333 | 801 |
| R5 | AAACGATACCCAGGTCCGCTCTC | 1,111-1,090 | |||
| F6a | ATGAGAATATTGGTACCCTGTTTCAGGGTCCGGTTTATTCTCCTATGTCTCG | zfs1 | NM_001022372.1 | 4-13 | 1212 |
| R6a | CTCGATTAACTAGTAGCTAGCTCAAGGAGATTGCTTAATAGTTG | 1,193-1,215 |
a
Underlined bases indicate zfs1 specific primer regions, whereas italic text highlights SspI and SpeI restriction sites for cloning into restriction enzyme-digested expression vector.