Table 1. Candidate antigens and selection criteria.

Gene	Locus tag	Fold change in vivo a	in vivo percentileb	Fold change in human urinec	Antigenicd	% UPEC (n = 55)e	% fecal (n = 30)e
chuA	c4308	7.06	95.0	27.8	+	87	30
hma	c2482	6.56	94.5	14.8	+	69	17
iha	c3610	18.9	89.1	5.87	+	45	37
ireA	c5174	23.3	95.2	7.81	+	20	17
iroN	c1250	22.7	82.7	7.63	+	71	33
iutA	c3623	5.57	97.0	49.2	+	65	17

^aTranscript fold change in the urine of experimentally infected mice as compared to growth in LB [29].

^bOf 5379 genes ranked in order of in vivo transcript microarray signal intensity [29].

^cProtein fold change following growth in pooled filter-sterilized human urine as compared to growth in LB [30].

^dReacts with sera from mice chronically infected with E. coli CFT073 [31].

^ePrevalence of indicated gene in UPEC and fecal isolates [31].