Table 2. The primer sequences used to PCR amplify the genes seq0936, seq0944, seq0256, seq0402, ideE2 and IdeE2.

Gene	Corresponding part in protein	Primer name	Sequence
seq0936	1–422	Eqp541	GCATCCATGGATACAGCAAGCTATACCA
		Eqp542	TCGACTCGAGGATAGACCCTTTTTTATTAAC
seq0944	1–427	Eqp271	GCAGCCATGGAGAGTCTGACGAGTGTTGA
		Eqp272	TCACCTCGAGTCCTAGCTCACCGTCATAAGC
seq0256	1–441	Eqp51	GTAGCCATGGAAACGACTACTGCTAGTGCA
		Eqp52	CTGGCTCGAGCGGTTTAGCAACCAAGGCT
seq0402	1–196	Eqp81	CATGCCATGGCGACTACCCTAGCAGGACAAA
		Eqp82	CTAGCTCGAGGTGCTTAAGCTTTTCAATCTG
IdeE	1–315	IdeG1	TACTGGATCCGACGATTACCAAAGGAATGCTAC
		IdeG2	TGATCTCGAGTTAGCTCAGTTTCTGCCATATG
IdeE2	1–344	Ide2F	CATGCCATGGAGGTAGTTGAAGTTTGGCCTAAT
		Ide2R	CCGCTCGAGTTTTTCTGTGTTGTTGAAGTAATCTGC

The bold nucleotides correspond to the introduced restriction cleavage sites. The number indicated in the corresponding protein refers to amino acids in the mature protein.